Gcg-icre
WebInternal positive control = 324 bp. The genotyping protocol (s) presented here have been … WebApr 7, 2024 · 78 containing glucagon promoter-driven iCRE (Tg(Gcg-icre) 12Fmgb. mice; see [29]). All mice were female 79. and fully backcrossed to a C57BL/6J background. Unless otherwise indicated animals had . ad . 80 . libitum. access to food and water. All animals were house in an SPF facility on a 12:12h light:dark 81. cycle at 22°C.
Gcg-icre
Did you know?
WebApr 27, 2024 · iCre. strain (33) was generated in the labo ratory of Dr. Gittes. ... b-cell mass, and serum glucagon (GCG) levels at the indicated time points during or after pregnancy. Data are. Webof transgenic GCG-iCre and INS-Cre-GCaMP6 mice, respectively, utilizing confocal and diSPIM imaging. in addition, we examine the effects of Com-plexin 2, a protein kinase A (PKA)-dependent SNARE binding protein, on the regulation of glucagon secretion, along with a-cell intracellular Ca2þ ac-
WebOct 1, 2024 · Heterozygous breeding was set up to produce in mice heterozygous for the Tg(Gcg-icre) 12Fmgb and the Gt(ROSA)26Sor tm38(CAG-GCaMP3)Hze allele. iCRE was always and only passed down through the father. All mice used in this study were 16–18 weeks old and fully backcrossed to a C57BL/6J background. Given the large differences … WebGcg-Cre rats are a novel and valuable experimental tool for analyzing the development, …
http://www.informatics.jax.org/recombinase/specificity?id=MGI:5529611&system=head WebSynonym: cresyn1, Syn-cre, Syn-Cretg, SynI-Cre. Molecular description: This transgene expresses Cre recombinase under the control of a rat synapsin I promoter. Cre recombinase activity is detected in neuronal cells, including brain, spinal cord and DRGs, as early as E12.5, as well as in neurons in adult. Line 671 contains 10 copies of the ...
WebGcg iCre mice express a codon improved Cre recombinase gene resulting in 93% to 95% recombination in pancreatic alpha cells (17). While the great majority of the cells that express preproglucagon...
WebQuantification of α- and β-cell Ca 2+ activities under glucose stimulation and various conditions were conducted in intact islets of transgenic GCG-iCre and INS-Cre-GCaMP6 mice, respectively, utilizing confocal and diSPIM imaging. in addition, we examine the effects of Complexin 2, a protein kinase A (PKA)-dependent SNARE binding protein, on ... chest x ray for pneumonia diagnosisWebThe genotyping protocol (s) presented here have been optimized for reagents and conditions used by The Jackson Laboratory (JAX). To genotype animals, JAX recommends researchers validate the assay independently upon receipt of animals into their facility. Reaction cycling temperature and times may require additional optimization based on the ... good shepherd lutheran church crandon wiWebOct 13, 2024 · Methods To visualize and manipulate Gcg -expressing cells in rats, CRISPR/Cas9 was used to express iCre under control of the Gcg promoter. Gcg-Cre rats were bred with tdTomato reporter rats to tag ... chest x ray for pneumothoraxWeb030542 B6.Cg- Gcg tm1.1(cre/GFP)Rpa /J These targeted mutant mice express cre recombinase from the mouse Gcg (glucagon) promoter in Gcg -expressing cells of the nucleus tractus solitarius (NTS). If you need help with your order, contact Customer Support. For … good shepherd lutheran church des plaines ilhttp://www.informatics.jax.org/recombinase/specificity?id=MGI:5490029&system=alimentary+system good shepherd lutheran church circle pines mnWebDownload scientific diagram Efficiency and selectivity of hindbrain iCre expression in Gcg-Cre/tdTom reporter rats. A, low magnification view of the hindbrain distribution of tdTom (red) and ... good shepherd lutheran church detroitchest x ray for ppd positive