WebMCF-7 cells were transfected with a reporter vector psiCHECK2-FSCN1 3'UTR or psiCHECK2 control vector and miR-133a mimic or negative control. Each transfection was carried out in triplicate. Luciferase assay was performed 48 h after gene transfection. Firefly luciferase activity was normalized to Renilla luciferase activity. WebDec 18, 2024 · Wild-type or mutant circCHST15 or 3′-UTR EIF4EBP1 was synthesized and then subcloned into psiCHECK-2 vector. HEK293T cells were seeded in 24-well plates at a concentration of 1.5 × 105 per well and cotransfected miR-125a-5p mimics or miR-NC with psiCHECK-2-wt-circCHST15, psiCHECK-2-mut-circCHST15, psiCHECK-2-wt-EIF4EBP1 or …

psiCHECK™-2 Vector - Promega

WebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … WebVector backbone psiCHECK-2 (Search Vector Database) Backbone manufacturer Promega Backbone size w/o insert (bp) 6273 Total vector size (bp) 6302 Vector type Mammalian Expression, Luciferase ; microRNA activity Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy … terapi bioresonansi alergi di malang

Dual Luciferase Reporter Vector Psicheck 2 Promega Bioz

WebpsiCHECK™-2: miR-21 Biosensor No light Figure 1. Detection of miR-21 activity using the psiCHECK™-2 Vector. miR-21 target sequence was cloned into the multiple cloning … WebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECK TM-2 vector, we found that seven of the eight sites in the Col4a3 – Col4a6 gene complementary to miR-29 were … terapi burung cipoh mp3